Chemical Composition and Biological Anti Microbial Activity of Some Extracts from Anemone Narcissiflora Subsp. Narcissiflora

Abstract

The antioxidant and antimicrobial activities of essential oils, methanolic and chloroform extracts from different parts of Anemone narcissiflora subsp. narcissiflora from Kumarganj Ayodhya were studied. Antioxidant activities were measured employing free radical, 2, 2-diphenyl- 1-picrylhydrazy1 (DPPH), scavenging ability of the samples. Total phenolic substance was measured for only methanol extracts. The extracts showed moderate free radical scavenging activity. Methanolic leaf extracts showed stronger inhibitory activity against DPPH redical with an SC50 values (the antioxidant concentration to achieve 50% radical scavenging) of 29 ug/mL. the antimicrobial activity was studied by the agar dilution minimal inhibitory concentration (MIC) assay and agar well diffusion assay using seven bacteria, Escherichia coli, Klebsiella pneumonia, Yersinia pseudotuberculosis, Pseudomonas aeruginosa, Enterococcus faecalis, staphylococcus aureus and Bacillus cereus, and two yeast-like fungi, candida tropicalis and candida tropicalis. Methanolic and chloroform extracts were effective against the bacteria except for B. Cereus and the fungi. The MIC values for the extracts ranged from 12.5 to 100 ug/mL, in many cases indicating better antibacterial activity as compared to the standard antibacterial ampicillin. In contract, the essential oils from the plant showed slight antimicrobial activity only on B. cereus at 500 ug/mL.