Validated Stability Indicating HPTLC of Aceclofenac

Authors

  • Dr. D. S. Ghotekar  Department of Chemistry, N.V.P.Mandal’s, Arts, Commerce and Science College Lasalgaon Tal-Niphad, Dist-Nashik, Maharashtra, India
  • Suvarna A Wagh  Department of Pharmaceutical chemistry, Maratha Vidya Prasarak Samaja’s Institute of Pharmaceutical Sciences„ Adgaon, Nashik, Maharashtra, India.
  • Pritishchandra S Kabra  Department of Pharmaceutics, DJPS College of pharmacy, pohetakli, Tal. pathri, Dist Parbhani, Maharashtra, India

Keywords:

Aceclofenac, validation, HPTLC

Abstract

The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for Aceclofenac in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for Aceclofenac (Rfvalue of 0.64 ? 0.028). Densitometric analysis of Aceclofenac was carried out in the absorbance mode at 243 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.999 with respect to peak area in the concentration range 30 - 120 ng/spot. The developed HPTLC method was validated with respect to accuracy, precision, recovery and robustness. Also to determine related substance and assay determination of Aceclofenac that can be used to evaluate the quality of regular production samples. The developed method can also be conveniently used for the assay determination of Aceclofenac. The limits of detection and quantitation were 4.062 and 12.322 ng/spot, respectively by height.

References

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International Journal of Scientific Research in Science, Engineering and Technology

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Published

2022-06-30

Issue

Volume 9, Issue 3, May-June-2022

Section

Research Articles

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How to Cite

[1]
Dr. D. S. Ghotekar, Suvarna A Wagh, Pritishchandra S Kabra, " Validated Stability Indicating HPTLC of Aceclofenac, International Journal of Scientific Research in Science, Engineering and Technology(IJSRSET), Print ISSN : 2395-1990, Online ISSN : 2394-4099, Volume 9, Issue 3, pp.441-447, May-June-2022.